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Western blot analysis of ALG10 Antibody in A375 cell line lysates (35ug/lane)
ALG10 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis of ALG10 Antibody in A375 cell line lysates (35ug/lane)

ALG10 Antibody

CATALOG NUMBER: 64-208

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This ALG10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-43 amino acids from the N-terminal region of human ALG10.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
56 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ALG10
Additional Names:
Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase, Alpha-1,2-glucosyltransferase ALG10-A, Alpha-2-glucosyltransferase ALG10-A, Asparagine-linked glycosylation protein 10 homolog A, ALG10, ALG10A
Protein Accession Number:
Q5BKT4
Protein Gi Number:
74736030
Ncbi Gene Id Number:
84920
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ALG10 encodes a membrane-associated protein that adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. That is, it transfers the terminal glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc2Man9GlcNAc(2)-PP-Dol. The rat protein homolog was shown to specifically modulate the gating function of the rat neuronal ether-a-go-go (EAG) potassium ion channel.
Background Reference 1:
Chapuis, J., et al. Mol. Psychiatry 14(11):1004-1016(2009)
Background Reference 2:
Seshadri, S., et al. BMC Med. Genet.SUPPL 1, S15 (2007)
Background Reference 3:
Kupershmidt, S., et al. FASEB J. 17(15):2263-2265(2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-208

List Size:
400 ul

List Price:

$495.00

Datasheet
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