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Western blot analysis of ALG14 Antibody in NCI-H460 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with ALG14 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of ALG14 Antibody in NCI-H460 cell line lysates (35ug/lane)

ALG14 Antibody

CATALOG NUMBER: 63-693

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ALG14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-93 amino acids from the Central region of human ALG14.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
24 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ALG14
Additional Names:
UDP-N-acetylglucosamine transferase subunit ALG14 homolog, ALG14
Protein Accession Number:
Q96F25
Protein Gi Number:
74731649
Ncbi Gene Id Number:
199857
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ALG14 is involved in protein N-glycosylation. It is essential for the second step of the dolichol-linked oligosaccharide pathway. It anchors the catalytic subunit ALG13 to the ER.
Background Reference 1:
Gao X.-D., et.al., J. Biol. Chem. 280:36254-36262(2005).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-693

List Size:
400 ul

List Price:

$495.00

Datasheet
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