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Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.

ALG2 Antibody

CATALOG NUMBER: 30-484

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ALG2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ALG2 antibody can be used for detection of ALG2 by ELISA at 1:312500. ALG2 antibody can be used for detection of ALG2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
47 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store ALG2 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
ALG2
Additional Names:
ALG2, CDGIi, FLJ14511, hALPG2, NET38
Protein Accession Number:
NP_149078
Protein Gi Number:
14861836
Ncbi Gene Id Number:
85365
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ALG2 is a member of the glycosyltransferase 1 family. It acts as an alpha 1,3 mannosyltransferase, mannosylating Man (2)GlcNAc (2)-dolichol diphosphate and Man (1)GlcNAc (2)-dolichol diphosphate to form Man (3)GlcNAc (2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii).This gene encodes a member of the glycosyltransferase 1 family. The encoded protein acts as an alpha 1,3 mannosyltransferase, mannosylating Man (2)GlcNAc (2)-dolichol diphosphate and Man (1)GlcNAc (2)-dolichol diphosphate to form Man (3)GlcNAc (2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Background Reference 1:
Draeby, I., (2007) Arch. Biochem. Biophys. 467 (1), 87-94.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-484

List Size:
100 ul

List Price:

$519.00

Datasheet
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