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Western blot analysis of ALK using rabbit polyclonal ALK Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ALK gene (Lane 2).
Formalin-fixed and paraffin-embedded human testis tissue reacted with ALK antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of ALK using rabbit polyclonal ALK Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ALK gene (Lane 2).

ALK Antibody

CATALOG NUMBER: 63-027

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ALK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 636-666 amino acids from the Central region of human ALK.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
176 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ALK
Additional Names:
ALK tyrosine kinase receptor, Anaplastic lymphoma kinase, CD246, ALK
Protein Accession Number:
Q9UM73
Protein Gi Number:
296439447
Ncbi Gene Id Number:
238
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ALK, a member of the insulin receptor subfamily of Tyr protein kinases, is an orphan receptor. It appears to play an important role in the normal development and function of the nervous system. This Type I membrane protein is expressed in brain and CNS and in the small intestine and testis, but not in normal lymphoid cells. A form of non-Hodgkin's lymphoma is characterized by a chromosomal translocation t(2;5)(p23;q35) that involves NPM1 and ALK. The protein contains 1 LDL-receptor class A domain and 2 putative MAM domains.
Background Reference 1:
Morris, S.W., et al., Oncogene 14(18):2175-2188 (1997).
Background Reference 2:
Iwahara, T., et al., Oncogene 14(4):439-449 (1997).
Background Reference 3:
Morris, S.W., et al., Science 263(5151):1281-1284 (1994).
Background Reference 4:
Morris, S.W., et al., Oncogene 15, 2883-2883 (1997).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-027

List Size:
400 ul

List Price:

$495.00

Datasheet
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