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Western blot analysis of extracts of 293T cells, using ALKBH1 antibody (15-361) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of U2OS cells using ALKBH1 antibody (15-361). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of 293T cells, using ALKBH1 antibody (15-361) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.

ALKBH1 Antibody

CATALOG NUMBER: 15-361

Clonality:
Polyclonal
Tested Applications:
IF, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 110-389 of human ALKBH1 (NP_006011.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IP, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:200
IP: 1:20 - 1:50
Positive Control 1:
293T
Predicted Molecular Weight:
Observed: 44kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ALKBH1
Additional Names:
ABH, ABH1, ALKBH, alkB, hABH, nucleic acid dioxygenase ALKBH1, DNA 6mA demethylase, DNA N6-methyl adenine demethylase, DNA demethylase ALKBH1, DNA lyase ABH1, DNA oxidative demethylase ALKBH1, alkB, alkylation repair homolog 1, alkylated DNA repair protein alkB homolog 1, alkylation repair, alkB homolog, alpha-ketoglutarate-dependent dioxygenase ABH1, tRNA N1-methyl adenine demethylase
Ncbi Gene Id Number:
8846
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a homolog to the E. coli alkB gene product. The E. coli alkB protein is part of the adaptive response mechanism of DNA alkylation damage repair. It is involved in damage reversal by oxidative demethylation of 1-methyladenine and 3-methylcytosine.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-361

List Size:
100 uL

List Price:

$436.00

Datasheet
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