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Western Blot at 1:1000 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
Western blot analysis in mouse cerebellum tissue lysates (35ug/lane).
ALKBH6 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.

ALKBH6 Antibody

CATALOG NUMBER: 55-757

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This ALKBH6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-145 amino acids from the Central region of human ALKBH6.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
26 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ALKBH6
Additional Names:
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 6, 11411-, Alkylated DNA repair protein alkB homolog 6, ALKBH6, ABH6
Protein Accession Number:
Q3KRA9
Protein Gi Number:
172046713
Ncbi Gene Id Number:
84964
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Probable dioxygenase that requires molecular oxygen, alpha-ketoglutarate and iron (By similarity).
Background Reference 1:
Strausberg, R.L., et al. Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-757

List Size:
400 ul

List Price:

$495.00

Datasheet
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