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Western Blot at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: MDA-MB-453 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in Hela(lane 1),A549(lane 2),K562(lane 3),MDA-MB435(lane 4) cell line lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human lymph tissue reacted with ALOX15 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Confocal immunofluorescent analysis of ALOX15 Antibody with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western Blot at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: MDA-MB-453 whole cell lysate Lysates/proteins at 20 ug per lane.

ALOX15 Antibody

CATALOG NUMBER: 63-276

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ALOX15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 610-643 amino acids from the C-terminal region of human ALOX15.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
75 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ALOX15
Additional Names:
Arachidonate 15-lipoxygenase, 15-LOX, 15-LOX-1, 12/15-lipoxygenase, Arachidonate 12-lipoxygenase, leukocyte-type, 12-LOX, Arachidonate omega-6 lipoxygenase, ALOX15, LOG15
Protein Accession Number:
P16050
Protein Gi Number:
126396
Ncbi Gene Id Number:
246
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ALOX15 converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. The protein also acts on C-12 of arachidonate as well as on linoleic acid.
Background Reference 1:
Tang,Y., Int. J. Cancer 124 (7), 1545-1551 (2009)
Background Reference 2:
Bevan,S., Stroke 40 (3), 696-701 (2009)
Background Reference 3:
Zuo,X., J. Biol. Chem. 283 (46), 31341-31347 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-276

List Size:
200 ul

List Price:

$495.00

Datasheet
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