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Western blot analysis of ALOX15B Antibody in CEM cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human skin tissue reacted with ALOX15B Antibody ,which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of ALOX15B Antibody in CEM cell line lysates (35ug/lane)

ALOX15B Antibody

CATALOG NUMBER: 63-834

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ALOX15B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 597-627 amino acids from the C-terminal region of human ALOX15B.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
76 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ALOX15B
Additional Names:
Arachidonate 15-lipoxygenase B, 15-LOX-B, 15-lipoxygenase 2, 15-LOX-2, Arachidonate 15-lipoxygenase type II, Linoleate 13-lipoxygenase 15-LOb, 11311-, ALOX15B
Protein Accession Number:
O15296
Ncbi Gene Id Number:
247
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ALOX15B encodes a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. The encoded protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. This gene is located in a cluster of related genes and a pseudogene that spans approximately 100 kilobases on the short arm of chromosome 17.
Background Reference 1:
Wecksler,A.T., et.al., Biochemistry 48 (36), 8721-8730 (2009)
Background Reference 2:
Kawakami,Y., et.al, Biosci. Biotechnol. Biochem. 73 (8), 1811-1817 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-834

List Size:
400 ul

List Price:

$495.00

Datasheet
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