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42-259 (1ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-259 (1ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Alpha 4GNT Antibody

CATALOG NUMBER: 42-259

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-PRTYRDLIKGPEGS
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 40kDa band observed in lysates of cell line K562 (calculated MW of 39.5kDa according to NP_057245.1). Recommended concentration: 1-3ug/ml.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
A4GNT
Additional Names:
A4GNT, alpha-1,4-N-acetylglucosaminyltransferase, MGC149493, alpha4GnT
Protein Accession Number:
NP_057245.1
Protein Gi Number:
7705859
Ncbi Gene Id Number:
51146
Background Reference 1:
Ishizone S, Yamauchi K, Kawa S, Suzuki T, Shimizu F, Harada O, Sugiyama A, Miyagawa S, Fukuda M, Nakayama J, Clinical utility of quantitative RT-PCR targeted to alpha1, 4-N-acetylglucosaminyltransferase mRNA for detection of pancreatic cancer. Cancer science 2006 Feb 97 (2): 119-26.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-259

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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