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<b> Flow-cytometry using the anti-Alpha-fetoprotein AF5 (</b> HepG2 cells were stained with unimmunized r antibody (black line) or the rmeric version of AF5 (24-244- blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.
<b> Western Blot using anti-Alpha-fetoprotein antibody AF5 (</b> HepG2 cell lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of AF5 (24-244) at 1 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Alpha-fetoprotein is 68.7kDa- though this protein is also glycosylated (Uniprot ID: P02771). 24-244 successfully detected human Alpha-fetoprotein in HepG2 cell lysates.
<b> Immunofluoresence staining of fixed HepG2 cells with anti-Alpha-fetoprotein (AFP) antibody AF5 (</b> Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells- permewith 0.15% Triton stained with the chimeric r version of AF5 (24-244) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-244- DAPI- merged channels and a negative control. The negative control was stained with unimmunized r followed by Alexa Fluor® 488 secondary antibody.
Flow-cytometry using the anti-Alpha-fetoprotein AF5 ( HepG2 cells were stained with unimmunized r antibody (black line) or the rmeric version of AF5 (24-244- blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.

Alpha-fetoprotein (AFP) Antibody [AF5], Rabbit IgG

CATALOG NUMBER: 24-244

Clonality:
Recombinant Monoclonal
Tested Applications:
Flow, IF, WB
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Species Reactivity:
Human
Species:
Rabbit
Original Species Name:
Mouse
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Immunogen:
AF5 was prepared by immunizing female BALB/c mice with human amniotic fluid containing AFP, from a pregnancy affected by a neural tube defect, diluted and emulsified in Freund's complete adjuvant.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, WB
Clonality:
Recombinant Monoclonal
Isotype:
IgG kappa
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Storage Conditions:
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Ncbi Official Symbol:
AFP
Additional Names:
A-Feto-protein
Ncbi Gene Id Number:
174
User Note:
For research use only.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

24-244

List Size:
0.2 mg

List Price:

$598.00

Datasheet
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