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Western blot analysis of lysates from ALPK1 knockout (KO) 293T and ALPK1 knockout (KO) 293T transfected with mouse ALPK1 using ALPK1 Rabbit mAb at 1:7000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 45s. | <b>WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS</b>
Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using ALPK1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded rat colon tissue using ALPK1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Western blot analysis of lysates from ALPK1 knockout (KO) 293T and ALPK1 knockout (KO) 293T transfected with mouse ALPK1 using ALPK1 Rabbit mAb at 1:7000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 45s. | WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS

ALPK1 Rabbit Recombinant Antibody (82-853)

CATALOG NUMBER: 82-853

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
WB,1:1000 - 1:7000, IHC-P,1:200 - 1:800, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
ALPK1 knockout (KO) 293T cells transfected with Mouse ALPK1
Predicted Molecular Weight:
139kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
Alpk1
Additional Names:
Alpha-kinase 1; ALPK1
Protein Accession Number:
E9QNX4
Ncbi Gene Id Number:
71481
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
α- Protein Kinase 1, also known as α- ALPK1 is associated with chronic kidney disease (CKD), myocardial infarction, gout and type 2 diabetes. According to reports, ALPK1 mutations can lead to serious deficits in motor coordination in mice. ALPK1 can enhance the expression of pro-inflammatory cytokines, including IL-1, in cultured human THP1 and human embryonic kidney 293 (HEK293) cells (PMID: 31557402, PMID: 26275947) β、 IL-8 and TGF- β 1. The ALPK1 short subtype (108 kDa) is highly expressed in the brain, spinal cord, heart, lungs, spleen, thymus, small intestine, skin, and testes, while detecting skeletal muscle and kidney. ALPK1 isoform (130 kDa) appears in the heart, lungs, thymus, and skin. As a novel innate immune receptor, ALPK1 can recognize bacteria-derived small molecule ADP-heptose and activate the immune response (PubMed: 30111836, PubMed: 30111836)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-853

List Size:
100 uL

List Price:

$540.00

Datasheet
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