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Western blot analysis in HepG2 cell line lysates (35ug/lane).
Western blot analysis of ALPP using rabbit polyclonal ALPP Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ALPP gene.
ALPP Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in HepG2 cell line lysates (35ug/lane).

ALPP Antibody

CATALOG NUMBER: 58-109

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ALPP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-83 amino acids from the N-terminal region of human ALPP.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
58 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ALPP
Additional Names:
Alkaline phosphatase, placental type, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1, ALPP, PLAP
Protein Accession Number:
P05187
Protein Gi Number:
130737
Ncbi Gene Id Number:
250
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
There are at least four distinct but related alkalinephosphatases: intestinal, placental, placental-like, andliver/bone/kidney (tissue non-specific). The first three arelocated together on chromosome 2 while the tissue non-specific formis located on chromosome 1. The product of this gene is a membranebound glycosylated enzyme, also referred to as the heat stableform, that is expressed primarily in the placenta although it isclosely related to the intestinal form of the enzyme as well as tothe placental-like form. The coding sequence for this form ofalkaline phosphatase is unique in that the 3' untranslated regioncontains multiple copies of an Alu family repeat. In addition, thisgene is polymorphic and three common alleles (type 1, type 2 andtype 3) for this form of alkaline phosphatase have been wellcharacterized.
Background Reference 1:
Stec, B., et al. Acta Crystallogr. Sect.Struct. Biol. Cryst. Commun. 66 (PT 8), 866-870 (2010) :
Background Reference 2:
Wang, F., et al. Am. J. Surg. Pathol. 33(10):1529-1539(2009)
Background Reference 3:
Estrada, K., et al. Hum. Mol. Genet. 18(18):3516-3524(2009)
Background Reference 4:
Zhu, J.F., et al. Zhonghua Wai Ke Za Zhi 47(5):381-384(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-109

List Size:
400 ul

List Price:

$495.00

Datasheet
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