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Western Blot at 1:1000 dilution Lane 1: human kidney lysate Lane 2: LNCaP whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
AMACR antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of AMACR Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western Blot at 1:1000 dilution Lane 1: human kidney lysate Lane 2: LNCaP whole cell lysate Lysates/proteins at 20 ug per lane.

AMACR Antibody

CATALOG NUMBER: 55-041

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This AMACR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 323-351 amino acids from the C-terminal region of human AMACR.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
42 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AMACR
Additional Names:
Alpha-methylacyl-CoA racemase, 2-methylacyl-CoA racemase, AMACR
Protein Accession Number:
Q9UHK6
Protein Gi Number:
313104070
Ncbi Gene Id Number:
23600
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described.
Background Reference 1:
Murray, N.P., et al. Oncol. Rep. 24(3):687-692(2010) Sonwalkar, S.A., et al. Histopathology 56(7):900-907(2010) Lakis, S., et al. World J. Gastroenterol. 16(20):2476-2483(2010) Chen, W., et al. Mol. Biol. Rep. 36(3):423-430(2009) Mubiru, J.N., et al. Gene 327(1):89-98(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-041

List Size:
400 ul

List Price:

$495.00

Datasheet
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