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Western blot analysis in HepG2 cell line lysates (35ug/lane).
AMBP Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in HepG2 cell line lysates (35ug/lane).

AMBP Antibody

CATALOG NUMBER: 57-253

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This AMBP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-104 amino acids from the N-terminal region of human AMBP.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
39 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AMBP
Additional Names:
Protein AMBP, Alpha-1-microglobulin, Protein HC, Alpha-1 microglycoprotein, Complex-forming glycoprotein heterogeneous in charge, Inter-alpha-trypsin inhibitor light chain, ITI-LC, Bikunin, EDC1, HI-30, Uronic-acid-rich protein, Trypstatin, AMBP, HCP, ITIL
Protein Accession Number:
P02760
Protein Gi Number:
122801
Ncbi Gene Id Number:
259
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a complex glycoprotein secreted inplasma. The precursor is proteolytically processed into distinctfunctioning proteins: alpha-1-microglobulin, which belongs to thesuperfamily of lipocalin transport proteins and may play a role inthe regulation of inflammatory processes, and bikunin, which is aurinary trypsin inhibitor belonging to the superfamily ofKunitz-type protease inhibitors and plays an important role in manyphysiological and pathological processes. This gene is located onchromosome 9 in a cluster of lipocalin genes.
Background Reference 1:
Olsson, M.G., et al. Radiat. Res. 174(5):590-600(2010)
Background Reference 2:
Allhorn, M., et al. Blood 99(6):1894-1901(2002)
Background Reference 3:
Amoresano, A., et al. Eur. J. Biochem. 267(7):2105-2112(2000)
Background Reference 4:
Xu, Y., et al. J. Mol. Biol. 276(5):955-966(1998)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-253

List Size:
400 ul

List Price:

$495.00

Datasheet
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