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Western blot analysis in K562 cell line lysates (35ug/lane).
AMELX Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in K562 cell line lysates (35ug/lane).

AMELX Antibody

CATALOG NUMBER: 56-898

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This AMELX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 amino acids from the N-terminal region of human AMELX.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
22 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AMELX
Additional Names:
Amelogenin, X isoform, AMELX, AMG, AMGX
Protein Accession Number:
Q99217
Protein Gi Number:
1168430
Ncbi Gene Id Number:
265
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the amelogenin family ofextracellular matrix proteins. Amelogenins are involved inbiomineralization during tooth enamel development. Mutations inthis gene cause X-linked amelogenesis imperfecta. Alternativesplicing results in multiple transcript variants encoding differentisoforms.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Almqvist, S., et al.Mater Sci Mater Med 21(3):947-954(2010)
Background Reference 3:
Lee, S.K., et al. J. Dent. Res. 89(1):46-50(2010)
Background Reference 4:
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-898

List Size:
400 ul

List Price:

$495.00

Datasheet
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