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Western blot analysis of AMFR using AMFR Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AMFR gene (Lane 2).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AMFR Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of HepG2 cells using AMFR Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of AMFR using AMFR Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AMFR gene (Lane 2).

AMFR Antibody

CATALOG NUMBER: 61-122

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This AMFR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 571-601 amino acids of human AMFR.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
73 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AMFR
Additional Names:
E3 ubiquitin-protein ligase AMFR, 632-, Autocrine motility factor receptor, AMF receptor, RING finger protein 45, gp78, AMFR, RNF45
Protein Accession Number:
Q9UKV5
Protein Gi Number:
34922250
Ncbi Gene Id Number:
267
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Autocrine motility factor (AMF) is a protein secreted by tumor cells that stimulates tumor motility. The gene for AMFR encodes a 323-amino acid polypeptide that has a single transmembrane domain and several putative glycosylation sites. The protein sequence has some homology to human tumor protein p53.
Background Reference 1:
Huang, B., et al., Biochem. Biophys. Res. Commun. 212(3):727-742 (1995).
Background Reference 2:
Watanabe, H., et al., J. Biol. Chem. 266(20):13442-13448 (1991).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-122

List Size:
400 ul

List Price:

$495.00

Datasheet
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