AMID Antibody

CATALOG NUMBER: 45-248

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: GSQVSVESGALHC
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 48-50kDa band in Human Colon and Duodenum lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 40.5kDa according to NP_116186. The 48-50kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle and Heart. Recommended concentration: 4ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
AIFM2
Additional Names:
AIFM2, AMID, PRG3, apoptosis-inducing factor (AIF)-like mitochondrion-associated inducer of death, 5430437E11Rik, p53-responsive gene 3, apoptosis-inducing factor (AIF)-homologous mitochondrion-associated inducer of death, apoptosis-inducing factor, mitochondrion-associated, 2, RP11-367H5.2, OTTHUMP00000019740, apoptosis-inducing factor (AIF)-homologous mitochondrion-associated inducer of death
Protein Accession Number:
NP_116186.1
Protein Gi Number:
14318424
Ncbi Gene Id Number:
84883
Background Reference 1:
Ohiro Y, Garkavtsev I, Kobayashi S, Sreekumar KR, Nantz R, Higashikubo BT, Duffy SL, Higashikubo R, Usheva A, Gius D, Kley N, Horikoshi N. A novel p53-inducible apoptogenic gene, PRG3, encodes a homologue of the apoptosis-inducing factor (AIF). FEBS Lett. 2002 Jul 31;524(1-3):163-71.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-248

List Size:
0.1 mg

List Price:

$495.00

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