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Western blot analysis of AMIGO1 in HeLa cell lysate with AMIGO1 antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of AMIGO1 in human brain tissue with AMIGO1 antibody at 5 μg/mL.
Immunofluorescence of AMIGO1 in human brain tissue with AMIGO1 antibody at 20 μg/mL.
Western blot analysis of AMIGO1 in HeLa cell lysate with AMIGO1 antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

AMIGO1 Antibody

CATALOG NUMBER: 7565

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
AMIGO1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human AMIGO1.
The immunogen is located within the last 50 amino acids of AMIGO1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
AMIGO1 antibody can be used for detection of AMIGO1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
AMIGO1 antibody is human, mouse, and rat reactive. AMIGO1 antibody is predicted to not cross-react with AMIGO2 or AMIGO3.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Predicted Molecular Weight:
Predicted: 54 kDa
Observed: 55 kDa
Purification:
AMIGO1 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
AMIGO1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
AMIGO1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
AMIGO1
Additional Names:
AMIGO1 Antibody: ALI2, AMIGO, AMIGO-1, ALI2, KIAA1163, Amphoterin-induced protein 1
Protein Accession Number:
NP_065754
Protein Gi Number:
153791466
Ncbi Gene Id Number:
57463
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The amphoterin-induced gene and ORF (AMIGO1) protein is a brain-enriched, glycosylated transmembrane immunoglobulin (Ig) superfamily protein with six extracellular leucine-rich repeats (LRRs) and one Ig-like domain. It and the related proteins AMIGO2 and AMIGO3 are thought to be cell adhesion molecules expressed on fiber tracts of neuronal tissues and participate in their formation (1). AMIGO1 has also been suggested to play important roles in dendritic outgrowth during development and could modulate the survival of developing and adult neurons (2).
Background Reference 1:
Kuja-Panula J, Kiiltomaki M, Yamashiro T, et al. AMIGO, a transmembrane protein implicated in axon tract development, defines a novel protein family with leucine-rich repeats. J. Cell Biol. 2003; 160:963-73.
Background Reference 2:
Chen Y, Hor HH, and Tang BL. AMIGO is expressed in multiple brain cell types and may regulate dendritic growth and neuronal survival. J. Cell Physiol. 2012; 227:2217-29.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7565

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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