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45-250 (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-250 (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Amisyn Antibody

CATALOG NUMBER: 45-250

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: SAKSAISKEIFAP-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave bands at both approx 25kDa and approx 45kDa in Human Brain extracts at 0.1ug/ml. Please note that currently we cannot find an explanation in the literature for the 45kDa band we observe given the predicted size of approx. 24Kda according to NP_054897. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Kidney shows cytoplasm staining of PCT and DCT cells Recommended concentration, 3-5ug/ml.
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Positive Control 2:
Cat. No. 10-401 - Human Kidney Tissue Slide
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
STXBP6
Additional Names:
STXBP6, syntaxin binding protein 6 (amisyn), amisyn, HSPC156, amisyn, syntaxin binding protein 6, FLJ39638, OTTHUMP00000178754
Protein Accession Number:
NP_054897.4
Protein Gi Number:
21426793
Ncbi Gene Id Number:
29091
Background Reference 1:
Scales SJ, Hesser BA, Masuda ES, Scheller RH. Amisyn, a novel syntaxin-binding protein that may regulate SNARE complex assembly. J Biol Chem. 2002 Aug 2;277(31):28271-9.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-250

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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