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Western blot analysis of extract from P19, HeLa and 293 cells using AML1 (RUNX1).
Western blot analysis of extract from P19, HeLa and 293 cells using AML1 (RUNX1).

AML1 (RUNX1) Antibody

CATALOG NUMBER: 79-674

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
AML1 (RUNX1) antibody was raised against a peptide sequence around aa.6~10 (D-A-S-T-S) derived from Human AML1 (RUNX1).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
This antibody detects endogenous level of total AML1(RUNX1) protein.
Predicted Molecular Weight:
55 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
RUNX1
Additional Names:
AML1, CBFA2, EVI-1, AMLCR1, PEBP2aB, AML1-EVI-1
Protein Accession Number:
NP_001116079.1
Protein Gi Number:
169790837
Ncbi Gene Id Number:
861
Background:
CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation.
Background Reference 1:
Mao S., Frank R. C., Zhang J., Miyazaki Y., Nimer S. D. Mol. Cell. Biol. 19:3635-3644 (1999) Pelletier N., Champagne N., Stifani S., Yang X. -J. Oncogene 21:2729-2740 (2002) Cho J. -Y., Akbarali Y., Zerbini L. F., Gu X., Boltax J., Wang Y., Oettgen P., Zhang D. -E., Libermann T. A. J. Biol. Chem. 279:19512-19522 (2004)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-674

List Size:
0.1 mg

List Price:

$468.00

Datasheet
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