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Western blot analysis of anti-AMPK alpha2 C-term Pab in mouse heart tissue lysate. AMPK alpha2 was detected using purified Pab.
Western blot analysis of anti-AMPK alpha2 C-term Pab in mouse heart tissue lysate. AMPK alpha2 was detected using purified Pab.

AMPK alpha2 Antibody

CATALOG NUMBER: 62-728

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This AMPK alpha2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-507 amino acids from the C-terminal region of human AMPK alpha2.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
62 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PRKAA2
Additional Names:
5'-AMP-activated protein kinase catalytic subunit alpha-2, AMPK subunit alpha-2, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, PRKAA2, AMPK, AMPK2
Protein Accession Number:
P54646
Protein Gi Number:
20178276
Ncbi Gene Id Number:
5563
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
AMPK2 alpha2 is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
Background Reference 1:
Minokoshi, Y., et al., Nature 428(6982):569-574 (2004).
Background Reference 2:
Andersson, U., et al., J. Biol. Chem. 279(13):12005-12008 (2004).
Background Reference 3:
Browne, G.J., et al., J. Biol. Chem. 279(13):12220-12231 (2004).
Background Reference 4:
Shaw, R.J., et al., Proc. Natl. Acad. Sci. U.S.A. 101(10):3329-3335 (2004).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-728

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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