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Antibody is used in Western blot to detect PRKAG1 in mouse heart tissue lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human testis tissue reacted with PRKAG1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Antibody is used in Western blot to detect PRKAG1 in mouse heart tissue lysate.

AMPK gamma Antibody

CATALOG NUMBER: 62-619

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Pig
Immunogen:
This AMPK gamma (PRKAG1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-295 amino acids from the Central region of human AMPK gamma (PRKAG1).
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
38 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PRKAG1
Additional Names:
5'-AMP-activated protein kinase subunit gamma-1, AMPK gamma1, AMPK subunit gamma-1, AMPKg, PRKAG1
Protein Accession Number:
P54619
Protein Gi Number:
1703037
Ncbi Gene Id Number:
5571
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PRKAG1 is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK.
Background Reference 1:
Minokoshi, Y., et al., Nature 428(6982):569-574 (2004).
Background Reference 2:
Hamilton, S.R., et al., FEBS Lett. 500(3):163-168 (2001).
Background Reference 3:
Zidovetzki, R., et al., AIDS Res. Hum. Retroviruses 14(10):825-833 (1998).
Background Reference 4:
Reinton, N., et al., Genomics 49(2):290-297 (1998).

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-619

List Size:
400 ul

List Price:

$495.00

Datasheet
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