Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of lysed extracts from A549, HepG2 and 3T3 cells using AMPKα1/AMPKα2 (Ab-174/172).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AMPKα1/AMPKα2 (Ab-174/172).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using AMPKα1/AMPKα2(Ab-174/172).
Western blot analysis of lysed extracts from A549, HepG2 and 3T3 cells using AMPKα1/AMPKα2 (Ab-174/172).

AMPKa1 / AMPKa2 (Ab 174 / 172) Antibody

CATALOG NUMBER: 79-357

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
AMPKa1/AMPKa2 (Ab-174/172) antibody was raised against a peptide sequence around aa. 172~176/170~174 (L-R-T-S-C) derived from Human AMPKα1/AMPKα2.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of total AMPKα1/AMPKα2 protein.
Predicted Molecular Weight:
63 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
PRKAA1 PRKAA2
Additional Names:
AMPK, AMPKa1, MAPTL, MTBT1, TAU,
Protein Accession Number:
NP_006242.5;NP_006243.2
Protein Gi Number:
94557301 / 46877068
Ncbi Gene Id Number:
5562;5563
Background:
Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
Background Reference 1:
Hurley RL, et al. (2005) J Biol Chem. Aug 12; 280 (32): 29060-29066Woods A, et al. (2003) Curr Biol. Nov 11; 13 (22): 2004-2008Nielsen JN, et al. (2003) J Appl Physiol. Feb; 94 (2): 631-641Da Silva Xavier G, et al. (2000) Proc Natl Acad Sci U S A. Apr 11; 97 (8): 4023-4028.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-357

List Size:
0.1 mg

List Price:

$370.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369