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Western blot analysis of AMY1A Antibody in HL-60 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with AMY1A Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of AMY1A Antibody in HL-60 cell line lysates (35ug/lane)

AMY1A Antibody

CATALOG NUMBER: 63-984

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Pig
Immunogen:
This AMY1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-223 amino acids from the Central region of human AMY1A.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
58 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AMY1A
Additional Names:
Alpha-amylase 1,4-alpha-D-glucan glucanohydrolase 1, Salivary alpha-amylase, AMY1A, AMY1
Protein Accession Number:
P04745
Protein Gi Number:
1351933
Ncbi Gene Id Number:
276277278
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. It is an amylase isoenzyme produced by the salivary gland.
Background Reference 1:
Ragunath, C., et al. J. Mol. Biol. 384(5):1232-1248(2008)
Background Reference 2:
Takahashi, T., et al. Neuro Endocrinol. Lett. 28(5):662-665(2007)
Background Reference 3:
Perry, G.H., et al. Nat. Genet. 39(10):1256-1260(2007)
Background Reference 4:
Chaudhuri, B., et al. BMC Microbiol. 7, 60 (2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-984

List Size:
400 ul

List Price:

$495.00

Datasheet
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