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Western blot analysis in HL-60,CEM and K562 cell line lysates (35ug/lane).
AMY2B antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of AMY2B Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis in HL-60,CEM and K562 cell line lysates (35ug/lane).

AMY2B Antibody

CATALOG NUMBER: 55-348

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This AMY2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74-100 amino acids from the N-terminal region of human AMY2B.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
58 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AMY2B
Additional Names:
Alpha-amylase 2B,4-alpha-D-glucan glucanohydrolase 2B, Carcinoid alpha-amylase, AMY2B
Protein Accession Number:
P19961
Protein Gi Number:
113789
Ncbi Gene Id Number:
280
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Amylases are secreted proteins that hydrolyze1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides,and thus catalyze the first step in digestion of dietary starch andglycogen. The human genome has a cluster of several amylase genesthat are expressed at high levels in either salivary gland orpancreas. This gene encodes an amylase isoenzyme produced by thepancreas.
Background Reference 1:
Baltova, S.D. Folia Med (Plovdiv) 47(1):37-41(2005)
Background Reference 2:
Fortna, A., et al. PLoS Biol.(7), E207 (2004) :
Background Reference 3:
Koyama, I., et al. Clin. Chim. Acta 309(1):73-83(2001)
Background Reference 4:
Aughsteen, A.A. Saudi Med22(5):410-415(2001)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-348

List Size:
400 ul

List Price:

$495.00

Datasheet
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