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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human ANGPT1 with DYKDDDDK tag probed with 42-372(1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 42-372 (1mg/ml) in Lane B. Primary
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human ANGPT1 with DYKDDDDK tag probed with 42-372(1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 42-372 (1mg/ml) in Lane B. Primary

ANGPT1 Antibody

CATALOG NUMBER: 42-372

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow, Pig
Immunogen:
The immunogen for this antibody is: C-PDFSSQKLQH
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:In transfected HEK293 transiently expressing full-length Human ANGPT1 (myc and DYKDDDDK tagged), a band of approx. 48kDa was observed. No bands were observed in mock-transfected HEK293 and the 48kDa plus a 75kDa band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 1-2ug/ml.Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email sales@everestbiotech.com to request datasheet, pricing and delivery information.
Positive Control 1:
Transfected HEK293
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ANGPT1
Additional Names:
ANGPT1, angiopoietin 1, AGP1, AGPT, ANG1
Protein Accession Number:
NP_001137.2
Protein Gi Number:
20532340
Ncbi Gene Id Number:
284

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-372

List Size:
0.1 mg

List Price:

$495.00

Datasheet
Shipping Info

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