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Western blot analysis in MDA-MB435,CEM,MCF-7,Jurkat,Hela cell line lysates (35ug/lane).This demonstrates the ANKFY1 antibody detected the ANKFY1 protein (arrow).
ANKFY1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in MDA-MB435,CEM,MCF-7,Jurkat,Hela cell line lysates (35ug/lane).This demonstrates the ANKFY1 antibody detected the ANKFY1 protein (arrow).

ANKFY1 Antibody

CATALOG NUMBER: 61-631

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ANKFY1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 436-465 amino acids from the C-terminal region of human ANKFY1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
128 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ANKFY1
Additional Names:
Rabankyrin-5, Rank-5, Ankyrin repeat and FYVE domain-containing protein 1, Ankyrin repeats hooked to a zinc finger motif, ANKFY1, ANKHZN, KIAA1255
Protein Accession Number:
Q9P2R3
Protein Gi Number:
33514905
Ncbi Gene Id Number:
51479
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ANKFY1 encodes a cytoplasmic protein that contains a coiled-coil structure and a BTB/POZ domain at its N-terminus, ankyrin repeats in the middle portion, and a FYVE-finger motif at its C-terminus. This protein belongs to a subgroup of double zinc finger proteins which may be involved in vesicle or protein transport.
Background Reference 1:
Bouslam, N., et al. Hum. Genet. 121 (3-4), 413-420 (2007)
Background Reference 2:
Schnatwinkel, C., et al. PLoS Biol.(9), E261 (2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-631

List Size:
400 ul

List Price:

$495.00

Datasheet
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