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46-949 (0.1ug/ml) staining of Human Substantia Nigra lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-949 (5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-949 (0.1ug/ml) staining of Human Substantia Nigra lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

ANKK1 Antibody

CATALOG NUMBER: 46-949

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-SRQADPNLHEAEGKT
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx. 85kDa band observed in Human Brain (Substantia nigra and Amygdala) lysates (calculated MW of 84.6 kDa according to NP_848605.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.An additional band of unknown identity was also consistently observed at 35 kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Prostate. Recommended concentration: 5-10ug/ml.
Positive Control 1:
Human Substantia Nigra Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ANKK1
Additional Names:
ANKK1, ankyrin repeat and kinase domain containing 1, PKK2, X-kinase, protein kinase PKK2
Protein Accession Number:
NP_848605.1
Protein Gi Number:
30425444
Ncbi Gene Id Number:
255239
Background Reference 1:
Dick DM, Wang JC, Plunkett J, Aliev F, Hinrichs A, Bertelsen S, Budde JP, Goldstein EL, Kaplan D, Edenberg HJ, Nurnberger J Jr, Hesselbrock V, Schuckit M, Kuperman S, Tischfield J, Porjesz B, Begleiter H, Bierut LJ, Goate A. Family-based association analyses of alcohol dependence phenotypes across DRD2 and neighboring gene ANKK1. Alcohol Clin Exp Res. 2007 Oct;31(10):1645-53.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-949

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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