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Western blot analysis of ANKLE2 in human testis tissue lysate with ANKLE2 antibody at (A) 1 and (B) 2 μg/mL.
Western blot analysis of ANKLE2 in human testis tissue lysate with ANKLE2 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

ANKLE2 Antibody

CATALOG NUMBER: 6609

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Immunogen:
ANKLE2 antibody was raised against a 19 amino acid synthetic peptide near the center of human ANKLE2.
The immunogen is located within amino acids 280 - 330 of ANKLE2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ANKLE2 antibody can be used for detection of ANKLE2 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Specificity:
Three isoforms of ANKLE2 are known to exist; this antibody will detect all three. ANKLE2 antibody is predicted to not cross-react with ANKLE1.
Positive Control 1:
Cat. No. 1313 - Human Testis Tissue Lysate
Predicted Molecular Weight:
Predicted: 121, 139, 145 kDa
Observed: 121 kDa
Purification:
ANKLE2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgY
physical-state:
Liquid
Buffer:
ANKLE2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ANKLE2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ANKLE2
Additional Names:
ANKLE2 Antibody: Lem4, LEMD7, KIAA0692, LEM4, Ankyrin repeat and LEM domain-containing protein 2, LEM domain-containing protein 4
Protein Accession Number:
AAH43157
Protein Gi Number:
148664230
Ncbi Gene Id Number:
23141
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ANKLE2 Antibody: Ankyrins are membrane adaptor molecules that play important roles in coupling integral membrane proteins to the spectrin-based cytoskeleton network. ANKLE2 (Ankyrin repeat and LEM domain containing 2) is a member of ankyrin repeat and LEM domain containing family. It is a potential single-pass membrane protein that contains three ankyrin repeats and a Lem domain. Ankyrin repeats mediate protein-protein interactions and are among the most common structural motifs in known proteins. The function of this protein is still under investigation.
Background Reference 1:
Lee KK and Wilson KL. All in the family: evidence for four new LEM-domain proteins Lem2 (NET-25), Lem3, Lem4 and Lem5 in the human genome. Symp. Soc. Exp. Biol. 2004; 56:329-39

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6609

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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