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45-255 (3.8ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-255 (3.8ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Annexin A10 Antibody

CATALOG NUMBER: 45-255

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Cow
Immunogen:
The immunogen for this antibody is: FCGDYVQGTIFPAPN
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments (at 1ug/ml) gave an approx 45-48kDa band in Hela, A431 and Human Kidney lysates. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 37kDa according to NP_009124. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Pancreas shows staining of islet of Langerhans . Recommended concentration, 3-5ug/ml.
Positive Control 1:
Cat. No. 1307 - Human Pancreas Tissue Lysate
Positive Control 2:
Cat. No. 11-101 - Human Pancreas Tissue Slide
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ANXA10
Additional Names:
ANXA10, annexin A10, ANX14, annexin 14
Protein Accession Number:
NP_009124.2
Protein Gi Number:
48762937
Ncbi Gene Id Number:
11199
Background Reference 1:
Morgan RO, Jenkins NA, Gilbert DJ, Copeland NG, Balsara BR, Testa JR, Fernandez MP. Novel human and mouse annexin A10 are linked to the genome duplications during early chordate evolution. Genomics. 1999 Aug 15;60(1):40-9.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-255

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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