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45-007 (0.01ug/ml) staining of HeLa (A) and Jurkat (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-007 (3.8ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-007 (0.01ug/ml) staining of HeLa (A) and Jurkat (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Annexin Antibody

CATALOG NUMBER: 45-007

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: YPGYPPPPGGYPP-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa band observed in lysates of cell lines HeLa and Jurkat (calculated MW of 54.4kDa according to NP_001148.1). Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:In paraffin embedded Human Placenta shows strong staining of Basement Membrane in trophoblasts. Recommended concentration, 3-5ug/ml.
Specificity:
Reported variants represent identical protein: NP_001148.1; NP_665875.1 and NP_665876.1.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ANXA11
Additional Names:
ANXA11, annexin A11, ANX11, CAP50, annexin XI, autoantigen, 56-kD, calcyclin-associated annexin 50, RP11-369J21, OTTHUMP00000019957, OTTHUMP00000019958,
Protein Accession Number:
NP_001148.1
Protein Gi Number:
4557317
Ncbi Gene Id Number:
311
Background Reference 1:
Misaki Y, Pruijn GJ, van der Kemp AW, van Venrooij WJ. The 56K autoantigen is identical to human annexin XI. J Biol Chem. 1994 Feb 11;269(6):4240-6.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-007

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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