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Western blot analysis of Anosmin in MCF7 cell lysate with Anosmin antibody at 1 μg/mL.
Immunocytochemistry of Anosmin in MCF7 cells with Anosmin antibody at 5 μg/mL.
Immunofluorescence of Anosmin in MCF7 cells with Anosmin antibody at 20 μg/mL.
Western blot analysis of Anosmin in MCF7 cell lysate with Anosmin antibody at 1 μg/mL.
Trial Size

Anosmin Antibody

CATALOG NUMBER: 6367

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Anosmin antibody was raised against an 18 amino acid synthetic peptide near the center of human Anosmin.
The immunogen is located within amino acids 430 - 480 of Anosmin.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
Anosmin antibody can be used for detection of Anosmin by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1219 - MCF7 Cell Lysate
Positive Control 2:
Cat. No. 17-012 - MCF7 Cell Slide
Purification:
Anosmin Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Anosmin Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Anosmin antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KAL1
Additional Names:
Anosmin Antibody: HH1, HHA, KAL, KMS, ADMLX, WFDC19, KALIG-1, KALIG1, Anosmin-1, Adhesion molecule-like X-linked
Protein Accession Number:
NP_000207
Protein Gi Number:
119395746
Ncbi Gene Id Number:
3730
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Anosmin Antibody: Mutations in Anosmin-1, an extracellular matrix-associated glycosylated protein, have been linked with Kallmann Syndrome (KS), an X-linked genetic disorder characterized by loss of smell caused by abnormal olfactory bulb development and delayed puberty caused by disrupted migration of the gonadotropin-releasing hormone neuron. Anosmin-1 has been shown to directly bind FGFR1 via its N-terminal cysteine-rich domain, whey-acidic protein-like domain, and its first FnIII repeat with the D2 and D3 ectodomains of FGFR1. It is thought that Anosmin-1 can modulate FGFR1 signaling and have opposing effects on the formation and activation of FGF2-FGFR1-heparing complex.
Background Reference 1:
Franco B, Guioli S, Pragliola A, et al. A gene deleted in Kallmann's syndrome shares homology with neural cell adhesion and axonal path-finding molecules. Nature 1991; 353:529-36.
Background Reference 2:
Soussi-Yanicostas N, Hardelin JP, Arroyo-Jimenez MM, et al. Initial characterization of anosmin-1, a putative extracellular matrix protein synthesized by definite neuronal cell populations in the central nervous system. J. Cell Sci. 1996; 109:1749-57.
Background Reference 3:
Hu Y, Guimond SE, Travers P, et al. Novel mechanisms of fibroblast growth factor receptor 1 regulation by extracellular matrix protein Anosmin-1. J. Biol. Chem. 2009; 284:29905-20

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6367

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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