Western blot analysis of extracts of various cell lines, using ANXA10 antibody (19-394) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

ANXA10 Antibody

CATALOG NUMBER: 19-394

Clonality:

Polyclonal

Tested Applications:

Host Species:

Rabbit

Species Reactivity:

Human

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 1-324 of human ANXA10 (NP_009124.2).

Conjugate:

Unconjugated

Tested Applications:

Application Note:

WB: 1:500 - 1:2000

Positive Control 1:

SGC-7901

Positive Control 2:

U-251MG

Positive Control 3:

BxPC-3

Predicted Molecular Weight:

Observed: 37kDa

Advanced Validation

Properties

Purification:

Affinity purification

Clonality:

Polyclonal

Isotype:

IgG

physical-state:

Liquid

Buffer:

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Concentration:

batch dependent

Storage Conditions:

Store at -20°C. Avoid freeze / thaw cycles.

Additional Info

Ncbi Official Symbol:

ANXA10

Additional Names:

ANXA10, annexin A10, ANX14, annexin 14

Ncbi Gene Id Number:

11199

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Background:

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. The function of this gene has not yet been determined.

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-394

List Size:

100 uL

List Price:

$436.00

Out of stock

Datasheet

Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

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ANXA10 Antibody

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Immunogen:

Conjugate:

Tested Applications:

Application Note:

Positive Control 1:

Positive Control 2:

Positive Control 3:

Predicted Molecular Weight:

Purification:

Clonality:

Isotype:

physical-state:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Ncbi Gene Id Number:

User Note:

Background:

Disclaimer:

List Size:

Shipping Info

Customize your order

Custom Antibody Services

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