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Western blot analysis of extracts of various cell lines, using ANXA2 Antibody (14-398) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts from normal (control) and ANXA2 knockout (KO) HeLa cells, using ANXA2 antibody (14-398) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.
Immunohistochemistry of paraffin-embedded rat heart using ANXA2 antibody (14-398) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using ANXA2 antibody (14-398) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using ANXA2 antibody (14-398) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using ANXA2 antibody (14-398). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using ANXA2 Antibody (14-398) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
KO

ANXA2 Antibody, KO Validated

CATALOG NUMBER: 14-398

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-339 of human ANXA2 (NP_004030.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:20 - 1:50
Positive Control 1:
SH-SY5Y
Positive Control 2:
HepG2
Positive Control 3:
Mouse lung
Predicted Molecular Weight:
Observed: 38kDa
Validation:
Antibody is Knockout validated.
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ANXA2
Additional Names:
ANXA2, P36, ANX2, LIP2, LPC2, CAL1H, LPC2D, ANX2L4, PAP-IV, HEL-S-270
Ncbi Gene Id Number:
302
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-398

List Size:
100 uL

List Price:

$436.00

Out of stock

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