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43-199 (0.1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-199 (0.1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

AOC3 / VAP1 Antibody

CATALOG NUMBER: 43-199

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: ESQAPKTIRDAFC
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 90kDa band observed in lysates of cell line A549 (calculated MW of 84.6kDa according to NP_003725.1). Recommended concentration: 0.1-0.3ug/ml.An additional band of unknown identity was also consistently observed at 38kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Predicted Molecular Weight:
Approx 90kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
AOC3 / VAP1
Additional Names:
AOC3, amine oxidase, copper containing 3 (vascular adhesion protein 1), HPAO, SSAO, VAP-1, VAP1, amine oxidase, copper containing 3, copper amine oxidase, semicarbazide-sensitive amine oxidase, vascular adhesion protein 1
Protein Accession Number:
NP_003725.1
Protein Gi Number:
4502119
Ncbi Gene Id Number:
8639
Background Reference 1:
Kiss J, Jalkanen S, Fülöp F, Savunen T, Salmi M, Ischemia-reperfusion injury is attenuated in VAP-1-deficient mice and by VAP-1 inhibitors.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-199

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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