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Western blot analysis of extracts of various cell lines, using AP1B1 antibody (16-538) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5min.
Western blot analysis of extracts of various cell lines, using AP1B1 antibody (16-538) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5min.

AP1B1 Antibody

CATALOG NUMBER: 16-538

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 700-800 of human AP1B1 (NP_001118.3).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
293T
Positive Control 2:
C6
Positive Control 3:
Mouse liver
Predicted Molecular Weight:
Observed: 105kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
AP1B1
Additional Names:
ADTB1, AP105A, BAM22, CLAPB2, AP-1 complex subunit beta-1, ADTB1, CLAPB2, Golgi adaptor HA1/AP1 adaptin beta subunit, adapter-related protein complex 1 subunit beta-1, adaptor protein complex AP-1 subunit beta-1, adaptor related protein complex 1 beta 1 subunit, beta-1-adaptin, beta-adaptin 1, beta-prime-adaptin, beta1-adaptin, clathrin assembly protein complex 1 beta large chain, plasma membrane adaptor HA2/AP2 adaptor beta subunit
Ncbi Gene Id Number:
162
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Adaptor protein complex 1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors. This complex is a heterotetramer composed of two large, one medium, and one small adaptin subunit. The protein encoded by this gene serves as one of the large subunits of this complex and is a member of the adaptin protein family. This gene is a candidate meningioma gene. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-538

List Size:
100 uL

List Price:

$436.00

Datasheet
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