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Western blot analysis of extracts of SW480 cells, using AP1S1 antibody (13-722) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
Western blot analysis of extracts of SW480 cells, using AP1S1 antibody (13-722) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.

AP1S1 Antibody

CATALOG NUMBER: 13-722

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human AP1S1 (NP_001274.1).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:1000
Positive Control 1:
SW480
Predicted Molecular Weight:
Observed: 19kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
AP1S1
Additional Names:
AP19, CLAPS1, EKV3, MEDNIK, SIGMA1A, AP-1 complex subunit sigma-1A, HA1 19 kDa subunit, adapter-related protein complex 1 sigma-1A subunit, adaptor protein complex AP-1 subunit sigma-1A, adaptor related protein complex 1 sigma 1 subunit, adaptor-related protein complex 1 subunit sigma-1A, clathrin assembly protein complex 1 sigma-1A small chain, clathrin coat assembly protein AP19, clathrin-associated/assembly/adaptor protein, small 1 (19kD), golgi adaptor HA1/AP1 adaptin sigma-1A subunit, sigma1A subunit of AP-1 clathrin adaptor complex, sigma1A-adaptin
Ncbi Gene Id Number:
1174
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is part of the clathrin coat assembly complex which links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. This protein, as well as beta-prime-adaptin, gamma-adaptin, and the medium (mu) chain AP47, form the AP-1 assembly protein complex located at the Golgi vesicle.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-722

List Size:
100 uL

List Price:

$419.00

Out of stock

Datasheet
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