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43-204 (0.01ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-204 (0.1ug/ml) staining of Mouse Brain lysate (~5ug protein in SDSPAGE buffer). The left panel shows the same blot stained with Ponceau red for total protein stain as the loading control before labelling. Primary incubation was 1 hour. Detected by che
43-204 (0.1ug/ml) staining of methanol-fixed HeLa cells with stably expressing Mouse Ap2a1. Primary incubation was 1 hour. Detected by Alexa Fluor 594. Data obtained from Prof. M Robinson, CIMR, Cambridge, UK.
43-204 (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-204 (0.01ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

AP2A1 Antibody

CATALOG NUMBER: 43-204

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-ELEPPAPESPMALLADPAPAAD
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 110kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 108kDa according to NP_055018.2). The same band is observed in Mouse Brain, but not in Mouse Liver or Mouse Kidney lysates. Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 5ug/ml.Immunocytochemistry: HeLa stably expressing a chimeric construct including the brain-specific splice variant of Mouse Ap2a1, showing clathrin-coated pits and vesicles budding from the plasma membrane.
Specificity:
This antibody is expected to recognize reported isoform 1 (NP_055018.2) only.
Predicted Molecular Weight:
Approx 110kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
AP2A1
Additional Names:
AP2A1, adaptor-related protein complex 2, alpha 1 subunit, ADTAA, AP2-ALPHA, CLAPA1, 100 kDa coated vesicle protein A, AP-2 complex subunit alpha-1, adapter-related protein complex 2 alpha-1 subunit, adaptin, alpha A, adaptor protein complex AP-2 subunit alpha-1, alpha-adaptin A, alpha1-adaptin, clathrin assembly protein complex 2 alpha-A large chain, clathrin-associated/assembly/adaptor protein, large, alpha 1, plasma membrane adaptor HA2/AP2 adaptin alpha A subunit
Protein Accession Number:
NP_055018.2
Protein Gi Number:
19913414
Ncbi Gene Id Number:
160
Background Reference 1:
Kitagawa Y, Kameoka M, Shoji-Kawata S, Iwabu Y, Mizuta H, Tokunaga K, Fujino M, Natori Y, Yura Y, Ikuta K.Inhibitory function of adapter-related protein complex 2 alpha 1 subunit in the process of nuclear translocation of human immunodeficiency virus type 1 genome.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-204

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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