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Western blot analysis in 293 cell line lysates (35ug/lane).
AP2A1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in 293 cell line lysates (35ug/lane).

AP2A1 Antibody

CATALOG NUMBER: 57-344

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This AP2A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 706-735 amino acids from the C-terminal region of human AP2A1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
108 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AP2A1
Additional Names:
AP-2 complex subunit alpha-1, 100 kDa coated vesicle protein A, Adaptor protein complex AP-2 subunit alpha-1, Adaptor-related protein complex 2 subunit alpha-1, Alpha-adaptin A, Alpha1-adaptin, Clathrin assembly protein complex 2 alpha-A large chain, Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit, AP2A1, ADTAA, CLAPA1
Protein Accession Number:
O95782
Ncbi Gene Id Number:
160
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes the alpha 1 adaptin subunit of theadaptor protein 2 (AP-2) complex found in clathrin coated vesicles.The AP-2 complex is a heterotetramer consisting of two largeadaptins (alpha or beta), a medium adaptin (mu), and a smalladaptin (sigma). The complex is part of the protein coat on thecytoplasmic face of coated vesicles which links clathrin toreceptors in vesicles. Alternative splicing of this gene results intwo transcript variants encoding two different isoforms. A thirdtranscript variant has been described, but its full length naturehas not been determined.
Background Reference 1:
Mitchell, R.S., et al. J. Virol. 82(16):7758-7767(2008)
Background Reference 2:
Kitagawa, Y., et al. Virology 373(1):171-180(2008)
Background Reference 3:
Stove, V., et al. J. Virol. 79(17):11422-11433(2005)
Background Reference 4:
Lu, W., et al. Neuron 47(3):407-421(2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-344

List Size:
400 ul

List Price:

$495.00

Datasheet
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