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Western blot analysis of extracts of various cell lines, using AP2B1 antibody (18-434) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human esophagus using AP2B1 Antibody (18-434) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using AP2B1 Antibody (18-434) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using AP2B1 Antibody (18-434) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using AP2B1 antibody (18-434) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

AP2B1 Antibody

CATALOG NUMBER: 18-434

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 752-951 of human AP2B1 (NP_001025177.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
22Rv1
Positive Control 2:
A-549
Positive Control 3:
K-562
Positive Control 4:
HeLa
Positive Control 5:
293T
Positive Control 6:
BT-474
Predicted Molecular Weight:
Observed: 105kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
AP2B1
Additional Names:
AP2B1, ADTB2, AP105B, AP2-BETA, CLAPB1, DKFZp781K0743
Ncbi Gene Id Number:
163
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. The encoded protein is found on the cytoplasmic face of coated vesicles in the plasma membrane. Two transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-434

List Size:
100 uL

List Price:

$436.00

Datasheet
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