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Western blot analysis in A2058 cell line lysates (35ug/lane).
Western blot analysis in A2058 cell line lysates (35ug/lane).

AP2B1 Antibody

CATALOG NUMBER: 58-131

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This AP2B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 517-546 amino acids from the Central region of human AP2B1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
105 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AP2B1
Additional Names:
AP-2 complex subunit beta, AP105B, Adaptor protein complex AP-2 subunit beta, Adaptor-related protein complex 2 subunit beta, Beta-2-adaptin, Beta-adaptin, Clathrin assembly protein complex 2 beta large chain, Plasma membrane adaptor HA2/AP2 adaptin beta subunit, AP2B1, ADTB2, CLAPB1
Protein Accession Number:
P63010
Protein Gi Number:
51702211
Ncbi Gene Id Number:
163
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is one of two large chaincomponents of the assembly protein complex 2, which serves to linkclathrin to receptors in coated vesicles. The encoded protein isfound on the cytoplasmic face of coated vesicles in the plasmamembrane. Two transcript variants encoding different isoforms havebeen found for this gene.
Background Reference 1:
Kahlfeldt, N., et al. J. Biol. Chem. 285(4):2734-2749(2010)
Background Reference 2:
Hood, F.E., et al. J. Cell. Sci. 122 (PT 13), 2185-2190 (2009) :
Background Reference 3:
Grass, B., et al. Histopathology 54(7):873-879(2009)
Background Reference 4:
Keyel, P.A., et al. Mol. Biol. Cell 19(12):5309-5326(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-131

List Size:
400 ul

List Price:

$495.00

Datasheet
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