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Western blot analysis of AP2M1 in human kidney tissue lysate with AP2M1 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of AP2M1 in mouse kidney tissue with AP2M1 antibody at 2.5 μg/mL.
Immunofluorescence of AP2M1 in Mouse Kidney cells with AP2M1 antibody at 20 μg/mL.
Western blot analysis of AP2M1 in human kidney tissue lysate with AP2M1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

AP2M1 Antibody

CATALOG NUMBER: 5161

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (100%)
Immunogen:
AP2M1 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human AP2M1.
The immunogen is located within amino acids 360 - 410 of AP2M1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
AP2M1 antibody can be used for detection of AP2M1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Purification:
AP2M1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgY
physical-state:
Liquid
Buffer:
AP2M1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
AP2M1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AP2M1
Additional Names:
AP2M1 Antibody: mu2, AP50, CLAPM1, KIAA0109, AP-2 complex subunit mu, AP-2 mu chain
Protein Accession Number:
NP_004059
Protein Gi Number:
14917109
Ncbi Gene Id Number:
1173
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
AP2M1 Antibody: The heterotetrameric coat assembly protein complex, also known as the adaptor-related protein complex 2 (AP-2), belongs to the adaptor complexes medium subunits family. The mu 1 subunit of the AP-2 complex (AP2M1) is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. AP2M1 has also been shown to associate with the HIV-1 protein Nef, suggesting that Nef may use AP-2 complex to enhance the rate of endocytosis of both CD4 and class I MHC. AP2M1 may also play an important role in regulating the intracellular trafficking and function of cytotoxic T-lymphocyte associated (CTLA)-4 protein. At least two isoforms of AP2M1 are known to exist.
Background Reference 1:
Robinson MS. Adaptable adaptors for coated vesicles. Trends Cell Biol.2004; 14:167-74.
Background Reference 2:
Aguilar RC, Ohno H, Roche KW, et al. Functional domain mapping of the clathrin-associated adaptor medium chains mu1 and mu J. Biol. Chem.1997; 272:27160-6.
Background Reference 3:
Liu Q, Feng Y, and Forgac M. Activity and in vitro reassembly of the coated vesicle (H+)-ATPase requires the 50-kDa subunit of the clathrin assembly complex AP-2. J. Biol. Chem.1994; 269:31592-7.
Background Reference 4:
Craig HM, Reddy TR, Riggs NL, et al. Interactions of HIV-1 Nef with the m subunits of adaptor protein complexes 1, 2, and 3: role of the dileucine-based sorting motif. Virology271:9-17.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5161

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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