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Western blot analysis of AP3B2 in rat brain tissue lysate with AP3B2 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of AP3B2 in mouse brain tissue with AP3B2 antibody at 5 μg/mL.
Immunofluorescence of AP3B2 in mouse brain tissue with AP3B2 antibody at 20 μg/mL.
Western blot analysis of AP3B2 expression in mouse brain tissue lysate with AP3B2 antibody at (A) 1 and (B) 2 μg/ml.
Western blot analysis of AP3B2 in rat brain tissue lysate with AP3B2 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

AP3B2 Antibody

CATALOG NUMBER: 6395

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
AP3B2 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human AP3B2.
The immunogen is located within the first 50 amino acids of AP3B2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
AP3B2 antibody can be used for detection of AP3B2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse and rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Positive Control 2:
Cat. No. 1403 – Mouse Brain Tissue Lysate
Predicted Molecular Weight:
Predicted: 121 kDa
Observed: 128 kDa
Purification:
AP3B2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
AP3B2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
AP3B2 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AP3B2
Additional Names:
AP3B2 Antibody: NAPTB, AP-3 complex subunit beta-2, Adapter-related protein complex 3 subunit beta-2
Protein Accession Number:
NP_001265440
Protein Gi Number:
34482047
Ncbi Gene Id Number:
8120
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
AP3B2 Antibody: The AP3 complex is a heterotetramer composed of two large adaptins (AP3D1, AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. Unlike the homologous AP3B1, AP3B2-containing AP3 complexes are preferentially targeted to neuronal processes, and mice deficient in AP3B2 displayed compromised synaptic zinc stores.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6395

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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