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Apaf-1 Antibody [2E12]

CATALOG NUMBER: 36-175

Clonality:
Monoclonal
Tested Applications:
ELISA, ICC, IHC, IP, WB
Host Species:
Rat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rat
Species Reactivity:
Human
Immunogen:
Recombinant human Apaf-1 (aa 1-464) containing the N-terminal CARD and CED-4 homologous domains.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IHC, IP, WB
Application Note:
Immunohistochemistry: (paraffin sections, 10ug/ml) . Immunohistochemistry: (EM on frozen sections, see G. Hausmann, et al.; (2000)) . Immunoprecipitation: (1:1000-1:2000) . Western Blot: (1:1000-1:2000) . Optimal conditions must be determined individually for each application.
Specificity:
Recognizes the CARD domain of human Apaf-1. Does not cross-react with mouse or rat Apaf-1.
Purification:
>95% (SDS-PAGE)
Clonality:
Monoclonal
Isotype:
IgG2a, kappa
physical-state:
Liquid
Buffer:
Liquid. In PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
Stable for at least 1 year after receipt when stored at -20°C.
Ncbi Official Symbol:
APAF1
Additional Names:
Apoptosis Protease-activating Factor 1
Protein Accession Number:
2330015
Protein Gi Number:
AAC51678
Ncbi Gene Id Number:
317
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

36-175

List Size:
100 ug

List Price:

$473.00

Datasheet
Shipping Info

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