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Western blot analysis of extracts of various cell lines, using APBB1 antibody (18-403) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human colon carcinoma using APBB1 antibody (18-403) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using APBB1 antibody (18-403). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using APBB1 antibody (18-403) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

APBB1 Antibody

CATALOG NUMBER: 18-403

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 359-708 of human APBB1 (NP_663722.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:50 - 1:200
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
Raji
Positive Control 2:
Lovo
Positive Control 3:
BT-474
Positive Control 4:
A-549
Positive Control 5:
Mouse brain
Positive Control 6:
Mouse heart
Predicted Molecular Weight:
Observed: 63kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
APBB1
Additional Names:
APBB1, amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65), FE65, MGC:9072, RIR, adaptor protein FE65a2, amyloid beta A4 precursor protein-binding, member 1, stat-like protein
Ncbi Gene Id Number:
322
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the Fe65 protein family. It is an adaptor protein localized in the nucleus. It interacts with the Alzheimer's disease amyloid precursor protein (APP), transcription factor CP2/LSF/LBP1 and the low-density lipoprotein receptor-related protein. APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. This encoded protein could play an important role in the pathogenesis of Alzheimer's disease. It is thought to regulate transcription. Also it is observed to block cell cycle progression by downregulating thymidylate synthase expression. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-403

List Size:
100 uL

List Price:

$436.00

Datasheet
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