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Western blot analysis of APC2 in HeLa cell lysate with APC2 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of APC2 in HeLa cells with APC2 antibody at 5 μg/mL.
Immunofluorescence of APC2 in HeLa cells with APC2 antibody at 20 μg/mL.
Western blot analysis of APC2 in HeLa cell lysate with APC2 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

APC2 Antibody

CATALOG NUMBER: 5721

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
APC2 antibody was raised against an 18 amino acid synthetic peptide near the center of human APC2.
The immunogen is located within amino acids 240 - 290 of APC2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
APC2 antibody can be used for detection of APC2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Purification:
APC2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
APC2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
APC2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
APC2
Additional Names:
APC2 Antibody: APCL, APCL, Adenomatous polyposis coli protein 2, Adenomatous polyposis coli protein-like, APC-like
Protein Accession Number:
BAA34611
Protein Gi Number:
3894265
Ncbi Gene Id Number:
10297
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
APC2 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC2 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. Like APC11, APC2 contains cullin and RING finger domains that are thought to be important in regulating ubiquitination activity.
Background Reference 1:
JM Peters. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol.2006; 7:644-56.
Background Reference 2:
Jorgensen PM, Graslund S, Betz R, et al. Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. Gene2001; 262:51-9.
Background Reference 3:
Yu H, Peters JM, King RW, et al. Identification of a cullin homology region in a subunit of the anaphase-promoting complex. Science1998; 279:1219-22.
Background Reference 4:
Zacharie W, Shevchenko A, Andrews PD, et al. Mass spectrometric analysis of the anaphase-promoting complex from yeast: identification of a subunit related to cullins. Science1998; 1216-19.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5721

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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