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Western blot analysis of APC6 in human liver tissue lysate with APC6 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of APC6 in human liver tissue with APC6 antibody at 5 μg/mL.
Immunofluorescence of APC6 in human liver tissue with APC6 antibody at 20 μg/mL.
Western blot analysis of APC6 in human liver tissue lysate with APC6 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

APC6 Antibody

CATALOG NUMBER: 5729

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
APC6 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human APC6.
The immunogen is located within the first 50 amino acids of APC6.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
APC6 antibody can be used for detection of APC6 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Positive Control 2:
Cat. No. 10-201 - Human Liver Tissue Slide
Purification:
APC6 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
APC6 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
APC6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CDC16
Additional Names:
APC6 Antibody: APC6, CUT9, ANAPC6, Cell division cycle protein 16 homolog, Anaphase-promoting complex subunit 6, APC6
Protein Accession Number:
NP_003894
Protein Gi Number:
118402580
Ncbi Gene Id Number:
8881
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
APC6 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC6 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. While little is known of APC6, it has been shown to interact with and is stabilized by CDC26 through an intermolecular TPR mimic composed of one helix from each protein.
Background Reference 1:
JM Peters. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol.2006; 7:644-56.
Background Reference 2:
Jorgensen PM, Graslund S, Betz R, et al. Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. Gene2001; 262:51-9.
Background Reference 3:
Wang J, Dye BT, Rajashankar KR, et al. Insights into anaphase promoting complex TPR subdomain assembly from a CDC26-APC structure. Nat. Struct. Mol. Biol.2009; 16:987-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5729

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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