APEX1 Antibody

CATALOG NUMBER: 27-662

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human APEX1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
APEX1 antibody can be used for detection of APEX1 by ELISA at 1:312500. APEX1 antibody can be used for detection of APEX1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
35 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store APEX1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
APEX1
Additional Names:
APEX1, APE, APX, APE1, APEN, APEX, HAP1, REF1
Protein Accession Number:
NP_542379
Protein Gi Number:
18375503
Ncbi Gene Id Number:
328
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells.
Background Reference 1:
Ding, S.Z., et al., (2004) Gastroenterology 127 (3), 845-858.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-662

List Size:
100 ul

List Price:

$473.00

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