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Western blot analysis of extracts of various cell lines, using APEX1 antibody (18-726).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts from normal (control) and APEX1 knockout (KO) 293T cells, using APEX1 antibody (18-726) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunofluorescence analysis of HeLa cells using APEX1 antibody (18-726). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of A549 cells using APEX1 antibody (18-726).
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug APEX1 antibody (18-726). Western blot was performed from the immunoprecipitate using APEX1 antibody (18-726) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using APEX1 antibody (18-726).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
KO

APEX1 Antibody, KO Validated

CATALOG NUMBER: 18-726

Clonality:
Polyclonal
Tested Applications:
IF, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-318 of human APEX1 (NP_542380.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IP, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:100
IP: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
HepG2
Predicted Molecular Weight:
Observed: 39kDa
Validation:
Antibody is Knockout validated.
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
APEX1
Additional Names:
APEX1, APE, APX, APE1, APEN, APEX, HAP1, REF1
Ncbi Gene Id Number:
328
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-726

List Size:
100 uL

List Price:

$540.00

Datasheet
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