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The purity of rh ApoA1 was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
The purity of rh ApoA1 was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.

Apo A1 Recombinant Protein

CATALOG NUMBER: 96-031

Tested Applications:
WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Arg 19 - Gln 267
Fusion Tag:
His Tag
Tested Applications:
WB
Application Note:
This recombinant protein can be used for WB. For research use only.
Predicted Molecular Weight:
29 kDa
Biological Activity:
Immobilized Human ApoAI at 10μg/mL (100μl/well) can bind biotinylated human SCARB1. The EC50 of biotinylated human SCARB1 is 10-100 ng/mL.
Purity:
>95% as determined by SDS-PAGE.
physical-state:
Lyophilized
Buffer:
PBS, pH7.4
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
APOA1
Additional Names:
Apolipoprotein A-I, APOA1, MGC117399
Protein Accession Number:
NP_000030.1
Ncbi Gene Id Number:
335
Background:
ApoA1 is also known as apolipoprotein A-I, ApoA-I , and is the major protein component of high density lipoprotein (HDL) in plasma. It has a specific role in lipid metabolism. Chylomicrons secreted from the intestinal enterocyte also contain ApoA1 but it is quickly transferred to HDL in the bloodstream. The protein promotes cholesterol efflux from tissues to the liver for excretion. It is a cofactor for lecithin cholesterol acyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. ApoA-I was also isolated as a prostacyclin (PGI2) stabilizing factor, and thus may have an anticlotting effect. Defects in the gene encoding it are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. In addition, it has been shown that ApoA1 is implicated in the anti-endotoxin function of HDL via interaction with lipopolysaccharide or endotoxin.
Background Reference 1:
Wasan KM , et al. 2008, Nature Reviews Drug Discovery 7 : 84–99.
Background Reference 2:
Yui Y, et al. 1988, J. Clin. Invest. 82 (3): 803–7.
Background Reference 3:
Eggerman, T.L. et al., 1991, J. Lipid Res. 32: 821-828.
Background Reference 4:
Voyiaziakis, E. et al., 1998. J. Lipid Res. 39: 313-321.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

96-031

List Size:
0.05 mg

List Price:

$540.00

Datasheet
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